武文强

武文强，男，博士，副教授，研究生导师，河南大学“青年英才”。主要从事单分子生物学、生物大分子结构动力学研究。2009-2017西北农林科技大学本博连读（创新实验班）。2017至今河南大学生命科学学院/省部共建作物逆境适应与改良国家重点实验室工作。曾获宝钢优秀学生奖、研究生国家奖学金、陕西省优秀博士学位论文、中原青年拔尖人才、河南省高层次人才、河南省优秀硕士论文指导教师、河南大学青年英才、教育部自然科学奖二等奖等荣誉。

主持项目：

1.国家自然科学基金面上项目（2022-2025）：植物特异解旋酶在RNA G-四链体介导mRNA成熟和翻译中的功能及分子机制（32171272），主持，在研

2.河南省自然科学基金优青项目（2024-2026）：植物G-四链体DNA特异解旋酶的功能及工作机制（242300421103），主持，在研

3.国家自然科学基金青年项目（2019-2021）：单分子水平研究G-四链体RNA的结构动力学及其与eIF4A的相互作用（31800644），主持，已结题

4.河南省高等学校重点科研项目（2019-2020）：用单分子方法研究拟南芥端粒G-四链体DNA结构动力学和解旋机制（19A180014），主持，已结题

主要论文：

第一作者(#)或通讯作者(*)

研究论文：

1. Zhang, B.^#,*, Li, S.-L.^#, Zhu, X., Yang, C.-S., Liu, Y., He, X., Zhou, P., Feng, T., Chen, L., Liu, Y., Dai, Y.-X., and Wu, W.-Q.* (2025) The stability and unfolding of DNA: RNA hybrid G-quadruplexes. Nucleic Acids Research, 53, 10.1093/nar/gkaf1182

2. Zhao, X. C.^#, Wu, B.^#, Yang, Y. J.^#, Li, Y.^#, Qiu, Q. Y.^#, Wang, L., Xu, Y. P., Gong, H., Song, L., Wang, X. J., Shi, J. Y., Chen, X. F., Zhang, S. R., Zong, Q., Dai, L., Du, S. S.*, Zhang, Y.*, Wu, W.-Q.*, and Zhang, X. H.* (2025) N6-methyladenine modification of DNA enhances RecA-mediated homologous recombination. Proc. Natl. Acad. Sci. U.S.A., 122, e2508652122

3. Zhang, C.^#, Tian, F.-J.^#, Zuo, H.-W.^#, Qiu, Q.-Y., Zhang, J.-H., Wei, W., Tan, Z.-J., Zhang, Y.*, Wu, W.-Q.*, Dai, L.*, and Zhang, X.-H.* (2025) Counterintuitive DNA destabilization by monovalent salt at high concentrations due to overcharging. Nature Communications, 16, 113.

4. Gao, C., Wei, S., Xu, Y., Mohamed, H. I., Liu, W., Wang, Z., Wu, W.-Q.*, Wang, M., and He, Y.* (2025) BRACO-19 targeted the G-quadruplex in the 3′UTR of the Cucumber mosaic virus 1a, 2b, and CP genes to inhibit viral proliferation. Pest Management Science, 10.1002/ps.8769

5. Guo, A.Y.^#, Wu, W.Q.^#, Bai, D., Li, Y., Xie, J., Guo, S. and Song, C.P.* (2024) Recruitment of HAB1 and SnRK2.2 by C2-domain protein CAR1 in plasma membrane ABA signaling. Plant Journal, 119, 237-251.

6. Guo, A.-Y.^#, Wu, W.-Q.^#*, Liu, W.-C., Zheng, Y., Bai, D., Li, Y., Xie, J., Guo, S. and Song, C.-P.* (2024) C2-domain abscisic acid-related proteins regulate the dynamics of a plasma membrane H⁺-ATPase in response to alkali stress. Plant Physiology, 196, 2784-2794.

7. Zhou, Y.^#, Zhang, T.^#, Wang, X.^#, Wu, W.Q.^#, Xing, J., Li, Z., Qiao, X., Zhang, C., Wang, X., Wang, G., Li, W., Bai, S., Li, Z., Suo, Y., Wang, J., Niu, Y., Zhang, J., Lan, C., Hu, Z., Li, B., Zhang, X., Wang, W., Galbraith, D. W., Chen, Y., Guo, S.*, and Song, C. P.* (2023) A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis. Nature Communications, 14, 4384.

8. Bai, D., Shan, S.-W., Zhang, X., Li, Y., Xie, J., and Wu, W.Q.* (2023) Comprehensive insights into the structures and dynamics of plant telomeric G-quadruplexes. International Journal of Biological Macromolecules, 231, 123281.

9. Wang, L.^#, Xu, Y.-P.^#, Bai, D., Shan, S.-W., Xie, J., Li, Y., and Wu, W.Q.* (2022) Insights into the structural dynamics and helicase-catalyzed unfolding of plant RNA G-quadruplexes. Journal of Biological Chemistry, 298, 102165.

10. Wu, W.Q.^#*, Zhang, X.^#, Bai, D., Shan, S.-W., and Guo, L.-J.* (2022) Mechanistic insights into poly(C)-binding protein hnRNP K resolving i-motif DNA secondary structures. Journal of Biological Chemistry, 298, 102670.

11. Wu, W.Q.^#, Zhang, M.L.^#, and Song, C.P.* (2020) A comprehensive evaluation of a typical plant telomeric G-quadruplex (G4) DNA reveals the dynamics of G4 formation, rearrangement, and unfolding. Journal of Biological Chemistry, 295, 5461-5469.

12. Xue, Z.Y.^#, Wu, W.Q.^#*, Zhao, X.C., Kumar, A., Ran, X., Zhang, X.H., Zhang, Y., and Guo, L.J.* (2020) Single-molecule probing the duplex and G4 unwinding patterns of a RecD family helicase.International Journal of Biological Macromolecules, 164, 902-910.

13. Zhang, M.L., Xu, Y.P., Kumar, A., Zhang, Y. and Wu, W.Q.* (2019) Studying the Potassium-Induced G-Quadruplex DNA Folding Process Using Microscale Thermophoresis. Biochemistry, 58, 3955-3959.

14. Wu, W.Q., Hou, X.M., Zhang , B, Fossé, P., René, B. and Mauffret, O., Li, M., Dou, S.X.* and Xi, X.G.* (2017) Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding. Scientific Reports, 7, 43954.

15. Zhang, B.^#, Wu, W.Q.^#, Liu, N.N., Duan, X.L., Li, M., Dou, S.X., Hou, X.M.* and Xi, X.G.* (2016) G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction. Nucleic Acids Research, 44, 8385-8394.

16. Wu, W.Q., Hou, X.M.*, Li, M., Dou, S.X. and Xi, X.G.* (2015) BLM unfolds G-quadruplexes in different structural environments through different mechanisms. Nucleic Acids Research, 43, 4614-4626.

17.Hou, X.M.^#, Wu, W.Q.^#, Duan, X.L., Liu, N.N., Li, H.H., Fu, J., Dou, S.X.*, Li, M. and Xi, X.G.* (2015) Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase. Biochemical Journal, 466, 189-199.

综述论文：

1. Zhang, Y.M., Li, B. and Wu, W.Q.* (2024) Single-molecule insights into repetitive helicases. Journal of Biological Chemistry, 300, 107894.

2. Guo, A.Y., Zhang, Y.M., Wang, L., Bai, D., Xu, Y.P., and Wu, W.Q.* (2021) Single-Molecule Imaging in Living Plant Cells: A Methodological Review. International Journal of Molecular Sciences, 22, 5071.

3. Wu, W.Q.*, Zhu, X. and Song, C.P.* (2019) Single-molecule technique: a revolutionary approach to exploring fundamental questions in plant science. New Phytologist, 223, 508-510.

主要荣誉：

1. 河南省高层次人才

2. 中原青年拔尖人才

3. 教育部自然科学奖二等奖

4. 河南省优青

5. 陕西省优秀博士学位论文

6. 河南省优秀硕士学位论文指导教师

7. 河南大学青年英才

8. 河南大学师德先进个人

9. 河南大学优秀研究生指导教师

社会兼职：

河南省生物物理学会副秘书长      

讲授课程：

1.分子生物学（本科生） 

2.高级植物生理（研究生）

3.文献阅读（研究生）         

E-mail:

wuwenqiang@henu.edu.cn

办公地点：

作物国重316